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The cytokeratin-18 (CK-18) fragment produced by neutrophil-derived proteases is a marker of neutrophil activation. In this study, we explored the potential of measuring CK-18 in blood as a marker of acute kidney injury (AKI) in critically ill patients. The first aim of this study was to define the kinetics of blood CK-18 in a porcine model of hemorrhagic shock. The second aim was to evaluate the value of CK-18 in predicting AKI in critically ill patients. CK-18 was measured in blood samples taken at various time points after the induction of shock in a porcine model. CK-18 was also measured in blood samples taken from patients admitted to a surgical intensive care unit (SICU), and the association of CK-18 with AKI was explored. Kinetics of CK-18 in blood were measured after the induction of shock in 8 pigs. Total CK-18, as determined by Western blotting, peaked 4 h after the induction of shock and was significantly higher (P < 0.001) compared to baseline values. In addition, there was a significant positive correlation between CK-18 and creatinine (r = 0.9, P < 0.001). The next aim of the study was to determine the value of CK-18 in predicting AKI in critically ill patients. This was done by measuring CK-18 in blood samples obtained from 205 patients, which were divided into a control group (n = 120) and an AKI group (n = 85). Patients in the AKI group were further divided into subgroups based on the timing of the AKI episode. CK-18 was significantly higher (P < 0.001) in the AKI group compared with controls. In addition, CK-18 was significantly higher (P < 0.001) in patients with AKI at any time point than in controls.

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Directed by: Ajay Jagdale. With: Kunal Kapoor, Jimmy Shergill, Kader Khan, Reema Lagoo, Farida Jalal, Iftekhar, Raj Babbar, Dharmendra, Kulbhushan Kharbanda, Nadira, Kunika, Rohini Hattangadi. IMDB rating is 6.4. Watch Bhoot and Friends on line with free movie streaming. Play or download Bhoot and Friends movie online full movie, Putlocker, Netflix, Fmovies, Openload, Viooz, MUBI, Megavideo, SinemArt, Netu, Vudu, Tunein, RnBx, BigMouth, Exeem, Veoh, Movie4k, CinemaNow.

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