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Quantifying the effect of diet on the structure of sugarcane rootborer, Sa. sabinae, was done through measuring the epicuticular wax (EW) in the head capsule of larva formed following feeding by four artificial diets. In the diet containing banana, wax was abundant in the posterior part of the head capsule. In the diet containing linseed, wax was abundant on dorsal and ventral surfaces of the head capsule. In the diet containing linseed and sorghum, wax was formed on the dorsal surface of the head capsule in addition to a ring. In the diet containing sugarcane, wax rings at different positions were observed. The diet containing banana stimulated the development of the mid-parts of the head capsule of the larva, while the diet containing linseed stimulated the production of a ring on the dorsal side. The diet containing linseed stimulated tar production, as well as the secretion of a large amount of wax from the larva to the diet. Tar was similar to a resin in that it was sticky and had a smaller grain size than wax. Wax has been recommended to be used in the development of various insecticides [9]. Tar and wax are potential candidates for a new insecticide, as they can efficiently change the physiology of pest insects. The newly formulated control method using banana and linseed is supposed to be efficient, but its mechanism should be investigated.
Out of the five different diets tested, it was determined that the diet containing linseed and sorghum and banana gave the most pest population. The structure of the wax in the larva differed depending on the diet, most noticeably in the abdomen. All the diets containing linseed were hairy with numerous large drops. In contrast, no wax was found in the mid-portions of the head capsule of the larva, which was formed by diet containing banana, as well as no drops of the mid-portion of the larva. Linseed and sorghum, as well as banana, are C4 grasses, and C4 species are rich in wax production, and linseed and sorghum are more productive than banana. However, the mid-portions of the head capsule of the larva formed by the diet containing banana had no wax, which is a disadvantage for crop protection as the main insecticide is to be placed in the insect mid-portion. d2c66b5586
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